Chromatin Dynamics and Cell Proliferation

Presentation

We study how chromatin dynamics govern cell fate/proliferation through:

a) Nucleosome-positioning, which controls the accessibility of key genomic features such as gene promoters/bodies or replication origins.

b) Higher-order chromatin folding for the regulation of transcription/replication, which involves DNA looping to mediate long-range interactions between distinct/functional elements.

We investigate how specific chromatin modifiers (Mes-4/NSD, Hypb etc.), regulators (insulator and co-factors), and oncogenes (dref, …) impact transcription or replication through chromatin dynamics.

To this end, we employ biochemistry, molecular genetics, cellular biology combined with computational biology of high throughput sequencing data to tackle key aspects of chromatin organization. 

https://www-lbme.biotoul.fr/uk/equipes/grpcuvier/index.html

 

 

Projets

HiCfeat

G4Hunter

Chromatin-based clonal inheritance

Chromatin dynamics

Publications

• Heurteau A, Perrois C, Depierre D, Fosseprez O, Humbert J, Schaak S, Cuvier O.
  Insulator-based loops mediate the spreading of H3K27me3 over distant micro-domains repressing euchromatin genes.
  Genome Biol.
  2020 Aug
• Farré P, Heurteau A, Cuvier O, Emberly E.
  Dense neural networks for predicting chromatin conformation.
  BMC Bioinformatics
  2018 Oct ; 19(1):372. doi: 10.1186/s12859-018-2286-z.
• Mourad R, Cuvier O.
  TAD-free analysis of architectural proteins and insulators.
  Nucleic Acids Res
  2018 Mar ; 46(5):e27. doi: 10.1093/nar/gkx1246.
• Mourad R, Ginalski K, Legube G, Cuvier O.
  Predicting double-strand DNA breaks using epigenome marks or DNA at kilobase resolution.
  Genome Biol.
  2018 Mar ; 19(1):34. doi: 10.1186/s13059-018-1411-7.
• Mourad R, Ginalski K, Legube G, Cuvier O.
  Predicting double-strand DNA breaks using epigenome marks or DNA at kilobase resolution
  Genome Biology
  2018 Mar

View all publications

Funding

       

Alumni

Raphaël Mourad (Maître de Conférence)

Jun Liang (Assistant Professor)

Priscillia Lhoumaud (Postdoctoral researcher)

Laurent Lacroix (Chargé de Recherche)

Franck Court (Chargé de Recherche)

Emmanuelle Guillou (Maître de Conférence)

Eldon Emberly (Associate Professor)

Pascal Martin (Ingénieur de Recherche)

Gael Micas

Sophie Queille