Publications
Publications de l'équipe Genevaux
- Mansour M, Giudice E, Xu X, Akarsu H, Bordes P, Guillet V, Bigot DJ, Slama N, D'urso G, Chat S, Redder P, Falquet L, Mourey L, Gillet R, Genevaux P.
Substrate recognition and cryo-EM structure of the ribosome-bound TAC toxin of Mycobacterium tuberculosis
Nature Communications
2022 May - Zhao L, Castanié-Cornet MP, Kumar S, Genevaux P, Hayer-Hartl M, Hartl FU..
Bacterial RF3 senses chaperone function in co-translational folding
Mol. Cell.
2021 Jun S1097-2765(21)00400-7. doi: 10.1016/j.molcel.2021.05.016. PMID: 34107307 - Bordes P, Genevaux P..
Control of Toxin-Antitoxin Systems by Proteases in Mycobacterium Tuberculosis
Front. Mol. Biosci.
2021 May 8:691399. doi: 10.3389/fmolb.2021.691399. eCollection 2021. PMID: 34079824 (Review) - Fauvet B, Finka A, Castanié-Cornet MP, Cirinesi AM, Genevaux P, Quadroni M, Goloubinoff P..
Bacterial Hsp90 Facilitates the Degradation of Aggregation-Prone Hsp70-Hsp40 Substrates
Front. Mol. Biosci.
2021 Apr 8:653073. doi: 10.3389/fmolb.2021.653073. PMID: 33937334 - Texier P, Bordes P, Nagpal J, Sala AJ, Mansour M, Cirinesi AM, Xu X, Dougan DA, Genevaux P.
ClpXP-mediated Degradation of the TAC Antitoxin is Neutralized by the SecB-like Chaperone in Mycobacterium tuberculosis
J Mol Biol. 433(5):166815. doi: 10.1016/j.jmb.2021.166815
2021 Jan Online ahead of print. PMID: 33450247 - Yiming Cai, Ben Usher, Claude Gutierrez, Anastasia Tolcan, Moise Mansour, Peter C. Fineran, Ciarán Condon, Olivier Neyrolles, Pierre Genevaux, Tim R. Blower.
A nucleotidyltransferase toxin inhibits growth of Mycobacterium tuberculosis through inactivation of tRNA acceptor stems
Science Advances
2020 Jul Vol. 6, no. 31, eabb6651 DOI: 10.1126/sciadv.abb6651 - Barriot R, Latour J, Castanié-Cornet MP, Fichant G, Genevaux P.
J-Domain Proteins in Bacteria and Their Viruses
J Mol Biol.
2020 Apr S0022-2836(20)30301-6. doi:10.1016/j.jmb.2020.04.014 - Ayala S, Genevaux P, Hureau C, Faller P.
(Bio)chemical Strategies To Modulate Amyloid-β Self-Assembly
ACS Chem. Neurosci
2019 Aug 10(8):3366-3374. doi:10.1021/acschemneuro.9b00239 - Akarsu H, Mansour M, Bordes P, Bigot DJ, Genevaux P, Falquet L.
TASmania: a bacterial Toxin-Antitoxin Systems database
PLoS Computational Biology
2019 Apr - Freire DM, Gutierrez C, Garza-Garcia A, Grabowska AD, Sala AJ, Ariyachaokun K, Panikova T, Beckham KSH, Colom A, Pogenberg V, Cianci M, Tuukkanen A, Boudehen YM, Peixoto A, Botella L, Svergun DI, Schnappinger D, Schneider TR, Genevaux P, de Carvalho LPS, Wilmanns M, Parret AHA and Neyrolles O.
An NAD+ phosphorylase toxin triggers Mycobacterium tuberculosis cell death
Mol. Cell
2019 Mar - Guillet V, Bordes P, Bon C, Marcoux J, Gervais V, Sala AJ, Dos Reis S, Slama N, Israel Mares-Mejía, Cirinesi AM, Maveyraud L, Genevaux P, Mourey L.
Structural insights into chaperone addiction of toxin-antitoxin systems
Nat. Commun.
2019 Feb - Kampinga HH, Andreasson C, Barducci A, Cheetham ME, Cyr D, Emanuelsson C, Genevaux P, Gestwicki JE, et al (+22 authors).
Function, evolution, and structure of J-domain proteins
Cell Stress Chaperones
2018 Nov - Texier P, Coddeville M, Bordes P, Genevaux P.
A useful gene cassette for conditional knock-down of essential genes by targeted promoter replacement in Mycobacteria
Biotechniques
2018 Sep - Mileo E, Ilbert M, Barducci A, Bordes P, Castanié-Cornet MP, Garnier C, Genevaux P, Gillet R, Goloubinoff P, Ochsenbein F, Richarme G, Iobbi-Nivol C, Giudici-Orticoni MT, Gontero B, Genest O.
Emerging fields in chaperone proteins: a French workshop
Biochimie
2018 Jun - Collet C, Thomassin J-L, Francetic O, Genevaux P, Tran Van Nhieu G.
Protein polarization driven by nucleoid exclusion of DnaK(HSP70)–substrate complexes
Nat. Commun
2018 May - Sala AJ, Bordes P, Ayala S, Slama N, Tranier S, Coddeville M, Cirinesi A-M, Castanié-Cornet M-P, Mourey L, and Genevaux P.
Directed evolution of SecB chaperones toward toxin-antitoxin systems
Proc. Natl. Acad. Sci. USA
2017 Nov - Bordes P, Genevaux P.
Addiction des systèmes toxine-antitoxine envers un chaperon chez Mycobacterium tuberculosis
Médecine & Sciences
2017 May - Fréderic Angles, Marie-Pierre Castanie-Cornet, Nawel Slama, Mickael Dinclaux, Anne-Marie Cirinesi, Jean-Charles Portais, Fabien Letisse and Pierre Genevaux.
Multilevel interaction of the DnaK/DnaJ(HSP70/HSP40) stress-responsive chaperone machine with the central metabolism
Sci. Rep
2017 Jan - Bordes P, Sala AJ, Ayala S, Texier P, Slama N, Cirinesi AM, Guillet V, Mourey L, Genevaux P.
Chaperone addiction of toxin-antitoxin systems
Nat Commun
2016 Nov - Schlegel S, Genevaux P, de Gier JW.
Isolating Escherichia coli strains for recombinant protein production
Cell Mol Life Sci
2016 Oct - Garcie C, Tronnet S, Garénaux A, McCarthy AJ, Brachmann AO, Pénary M, Houle S, Nougayrède JP, Piel J, Taylor PW, Dozois CM, Genevaux P, Oswald E, Martin P.
The Bacterial Stress-Responsive Hsp90 Chaperone (HtpG) Is Required for the Production of the Genotoxin Colibactin and the Siderophore Yersiniabactin in Escherichia coli
J Infect Dis
2016 Sep - Ruiz-González MX, Czirják GÁ, Genevaux P, Møller AP, Mousseau TA, Heeb P..
Resistance of Feather-Associated Bacteria to Intermediate Levels of Ionizing Radiation near Chernobyl
Sci. Rep
2016 Mar - Schlegel S, Genevaux P, de Gier J-W.
De-convoluting the Genetic Adaptations of E. coli C41(DE3) in Real Time Reveals How Alleviating Protein Production Stress Improves Yields
Cell Rep
2015 Mar - Sala A, Bordes P, Genevaux P.
Multitasking SecB chaperones in bacteria
Front. Microbiol
2014 Dec - Castanié-Cornet MP, Bruel N, Genevaux P.
Chaperone networking facilitates protein targeting to the bacterial cytoplasmic membrane
Special issue SI: Protein trafficking & Secretion in Bacteria. BBA - Molecular Cell Research
2014 Aug - Götzke H, Palombo I, Muheim C, Perrody E, Genevaux P, Kudva R, Müller M, Daley OD.
YfgM is an Ancillary Subunit of the SecYEG Translocon in Escherichia coli
J. Biol. Chem
2014 May - Sala A, Bordes P, Genevaux P.
Multiple toxin-antitoxin systems in Mycobacterium tuberculosis
Toxins
2014 Mar - Sala A, Calderon V, Bordes P, Genevaux P.
TAC from Mycobacterium tuberculosis: a paradigm for toxin-antitoxin systems controlled by SecB-like chaperones
Cell Stress and Chaperones
2013 Dec - Sala A, Bordes P, Fichant G, Genevaux P.
Toxin-antitoxin systems in Mycobacterium tuberculosis
In Prokaryotic Toxin-Antitoxins, Springer, Kenn Gerdes (Ed.)
2013 Jan - Perrody E, Cirinesi AM, Desplats C, Keppel F, Schwager F, Tranier S, Georgopoulos C, Genevaux P.
A Bacteriophage-Encoded J-Domain Protein Interacts with the DnaK/Hsp70 Chaperone and Stabilizes the Heat-Shock Factor σ32 of Escherichia coli
PLoS Genet
2012 Nov - Calloni G, Chen T, Schermann SM, Chang HC, Genevaux P, Agostini F.
DnaK Functions as a Central Hub in the E. coli Chaperone Network
Cell Rep
2012 Mar - Bruel N, Castanié-Cornet MP, Cirinesi AM, Koningstein G, Georgopoulos C, Luirink J, Genevaux P.
Hsp33 controls Elongation Factor-Tu stability and allows Escherichia coli growth in the absence of the major DnaK and Trigger Factor chaperones
J. Biol. Chem
2012 Jan - Champ S, Puvirajesinghe TM, Perrody E, Menouni R, Genevaux P, Ansaldi M.
Chaperone-assisted excisive recombinantion, a solitary role for DnaJ (Hsp40) chaperone in lysogeny escape
J. Biol. Chem
2011 Jul - Bordes P, Cirinesi AM, Ummels R, Sala A, Sakr S, Bitter W, Genevaux P.
SecB-like chaperone controls a toxin-antitoxin stress-responsive system in Mycobacterium tuberculosis
Proc. Natl. Acad. Sci. USA
2011 May - Maillard J, Genevaux P, Holliger C.
Redundancy and specificity of multiple trigger factor chaperones in Desulfitobacteria
Microbiology
2011 May - Maddalo G, Stenberg-Bruzell F, Götzke H, Toddo S, Björkholm P, Eriksson H, Chovanec P, Genevaux P, Lehtiö J, Ilag LL, Daley DO.
Systematic Analysis of Native Membrane Protein Complexes in Escherichia coli
J. Proteome Res
2011 Jan - Jong WS, Ten Hagen-Jongman CM, Ruijter E, Orru RV, Genevaux P, Luirink J.
YidC is involved in the biogenesis of the secreted autotransporter Hbp
J. Biol. Chem
2010 Oct - Horne BE, Li T, Genevaux P, Georgopoulos C, Landry SJ.
The Hsp40 J-domain stimulates Hsp70 when tethered by the client to the ATPase domain
J. Biol. Chem
2010 May - Sakr S, Cirinesi AM, Ullers RS, Schwager F, Georgopoulos C, Genevaux P.
Lon protease quality control of presecretory proteins in Escherichia coli and its dependence on the SecB and DnaJ (Hsp40) chaperones
J. Biol. Chem
2010 May - Wagner S, Pop O, Haan GJ, Baars L, Koningstein G, Klepsch MM, Genevaux P, Luirink J and de Gier JW.
Biogenesis of MalF and the MalFGK(2) maltose transport complex in Escherichia coli requires YidC
J. Biol. Chem
2008 May - Genevaux P, Georgopoulos C and Kelley WL.
The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions
Mol. Microbiol
2007 Oct - Ullers R, Genevaux P and Luirink J.
Cotranslational protein targeting in Escherichia coli
In The Enzymes Vol. XXV. Academic Press. Edited by Dalbey RE, Koehler CM and Tamanoi F
2007 Oct - Ullers RS, Ang D, Schwager F, Georgopoulos C and Genevaux P.
Trigger Factor can antagonize both SecB and DnaK/DnaJ chaperone functions in Escherichia coli
Proc. Natl. Acad. Sci. USA
2007 Feb - 2007 Jan Tu Quoc PH, Genevaux P, Pajunen M, Savilahti H, Georgopoulos C, Schrenzel J and Kelley WL. 2007. Isolation and characterization of biofilm formation-defective mutants of Staphylococcus aureus. Infect. Immun. 75:1079-1088.
- 2007 Jan Lakshmipathy SK, Tomic S, Kaiser CM, Chang HC, Genevaux P, Georgopoulos C, Barral JM, Johnson AE, Hartl FU, Etchells SA. 2007. Identification of nascent chain interaction sites on Trigger Factor. J. Biol. Chem. 282:12186-12193.
- 2006 Jan Cogelja Cajo G, Kelley WL, Schwager F, Georgopoulos C and Genevaux P. 2006. The role of the conserved DIF repeats of the DnaJ (Hsp40) co-chaperone in the regulation of the DnaK (Hsp70) chaperone cycle. J. Biol. Chem. 281:12436-12444.
- 2006 Jan Maranic P, Wagnera S, Baars L, Genevaux P, de Gier J-W, Nilsson I-G, Casadioc R, von Heijne G. 2006. New Escherichia coli outer membrane proteins identified through prediction and experimental verification. Protein Sci. 14:884-889.
- 2005 Jan Drew D, Slotboom D-J, Friso G, Reda T, Genevaux P, van Wijk K-J, Hirst J, Kunji E and de Gier J-W. 2005. A scalable, GFP-based pipeline for membrane protein overexpression screening and purificatioon. Protein Sci. 14:2011-2017.
- 2004 Jan Ullers R, Luirink J, Harms N, Schwager F, Georgopoulos C and Genevaux P. 2004. SecB is a bona fide generalized chaperone in Escherichia coli. Proc. Natl. Acad. Sci. USA. 101:7583-7588.
- 2004 Jan Jong WSP, ten Hagen-Jongeman CM, Genevaux P, Brunner J, Oudega B and Luirink J. 2004. Trigger Factor interacts with the signal peptide of the nascent Tat substrates but does not play a critical role in Tat-mediated export. Euro. J. Biochem. 271:4779-4787.
- 2004 Jan Gur E, Brian D, Genevaux P, Georgopoulos C and Ron EZ. 2004. The Escherichia coli DjlA and CbpA proteins can substitute for DnaJ in DnaK-mediated protein disaggregation. J. Bacteriol. 186:7236-7242.
- 2004 Jan Genevaux P, Keppel F, Schwager F, Langendijk-Genevaux PS, Hartl FU and Georgopoulos C. 2004. In vivo dissection of Trigger Factor and DnaK overlapping functions. EMBO Rep. 5:195-200.
- 2004 Jan Agashe VR, Guha S, Chang H-C, Genevaux P, Hayer-Hartl M, Stemp M, Georgopoulos C,. Hartl FU and Barral JM. 2004. Function of Trigger Factor and DnaK in multidomain protein folding: Increase in yield at the expense of folding speed. Cell. 117:199-209.
- 2003 Jan Genevaux P, Lang F, Schwager F, Vatikar JV, Rundell K, Pipas JM. Georgopoulos C and Kelley WL. 2003. Simian Virus 40 (SV40) T antigens and J-domains: analysis of Hsp40 co-chaperone functions in Escherichia coli. J. Virol. 77:10706-10713.
- 2003 Jan Sardesai A, Genevaux P, Schwager F, Ang D and Georgopoulos C. 2003. The OmpL porin does not modulate redox potential in the periplasmic space of Escherichia coli. EMBO. J. 22:1461-1566.
- 2002 Jan Genevaux P, Schwager F, Georgopoulos C and Kelley WL. 2002. Scanning mutagenesis identifies amino acid residues essential for the in vivo activity of the Escherichia coli DnaJ (Hsp40) J-domain. Genetics 162:1045-1053.
- 2002 Jan Kluck CJ, Patzelt H, Genevaux P, Brehmer D, Bukau B and Mayer MP. 2002. Characterization of HscC, a specialized Hsp70 chaperone of Escherichia coli. J. Biol. Chem. 227:41060-41069.
- 2001 Jan Genevaux P, Schwager F, Georgopoulos C and Kelley WL. 2001. The djlA gene acts synergistically with dnaJ in promoting Escherichia coli growth. J. Bacteriol. 183:5747-5750.
- 2001 Jan Genevaux P, Wawrzynow A, Zylicz M, Georgopoulos C and Kelley WL. 2001. DjlA is a third DnaK co-chaperone of Escherichia coli and DjlA-mediated induction of colanic acid capsule requires DjlA-DnaK interaction. J. Biol. Chem. 276:7906-7912.
