Publications
Publications de l'équipe Polard
- Calum H G Johnston, Rachel Hope, Anne-Lise Soulet, Marie Dewailly, David De Lemos, Patrice Polard.
The RecA-directed recombination pathway of natural transformation initiates at chromosomal replication forks in the pneumococcus
Proceedings of the National Academy of Sciences (PNAS)
2023 Feb - Maud Hertzog, Thomas Noé Perry, Pauline Dupaigne, Sandra Serres, Violette Morales, Anne-Lise Soulet, Jason C Bell, Emmanuel Margeat, Stephen C Kowalczykowski, Eric Le Cam, Rémi Fronzes, Patrice Polard.
Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae
Nucleic Acids Research
2023 Feb - Vanessa Khemici, Marc Prudhomme, Patrice Polard.
Tight Interplay between Replication Stress and Competence Induction in Streptococcus pneumoniae
Cells
2021 Jul - Johnston CHG, Soulet AL, Bergé M, Prudhomme M, De Lemos D, Polard P..
The alternative sigma factor σX mediates competence shut-off at the cell pole in Streptococcus pneumoniae
eLife
2020 Nov - Jun Kurushima, Nathalie Campo, Renske van Raaphorst, Guillaume Cerckel, Patrice Polard, Jan-Willem Veening.
Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events
Elife
2020 Sep - Isabelle Mortier-Barrière, Patrice Polard, Nathalie Campo.
Direct Visualization of Horizontal Gene Transfer by Transformation in Live Pneumococcal Cells Using Microfluidics
Genes
2020 Jun - De Ste Croix M, Chen K, Vacca I, Manso A, Johnston C, Polard P, Kwun M, Bentley S, Croucher N, Bayliss C, Haigh R, Oggioni M..
Recombination of the Phase-Variable spnIII Locus Is Independent of All Known Pneumococcal Site-Specific Recombinases.
Journal of Bacteriology
2019 Jul - Isabelle Mortier-Barrière, Nathalie Campo, Mathieu A Bergé, Marc Prudhomme, Patrice Polard.
Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome
Methods Mol Biol
2019 Mar - Ramírez-Guadiana F, Rodrigues C, Marquis K, Campo N, Barajas-Ornelas R, Brock K, Marks D, Kruse A, Rudner D..
Evidence that regulation of intramembrane proteolysis is mediated by substrate gating during sporulation in Bacillus subtilis.
PLoS Genetics
2018 Nov - Johnston, C. Mortier-Barriere, I., Khemici, V. Polard, P..
Fine-tuning cellular levels of DprA ensures transformant fitness in the human pathogen Streptococcus pneumoniae.
Molecular Microbiology
2018 Sep - Weyder M, Prudhomme M, Bergé M, Polard P, Fichant G..
Dynamic Modeling of Streptococcus pneumoniae Competence Provides Regulatory Mechanistic Insights Into Its Tight Temporal Regulation
Front Microbiol
2018 Jul 9:1637. doi:10.3389/fmicb.2018.01637. - Weyder, M. Prudhomme, M. Berge, M, Polard, P. Fichant, G..
Dynamic Modeling of Streptococcus pneumoniae Competence Provides Regulatory Mechanistic Insights Into Its Tight Temporal Regulation.
Frontiers in Microbiology
2018 Jul - Bonnet, J. Durmort, C. Mortier-Barriere, I. Campo, N. JacQ, M. Moriscot, C. Straume, D. Berg, K. Wong, Y. Havarstein, L. Vernet, T. Di Guilmi, AM..
Nascent teichoic acids insertion into the cell wall directs the localization and activity of the major pneumococcal autolysin LytA
The Cell Surface
2018 Jun - Thomas Germier, Sylvain Audibert, Silvia Kocanova, David Lane and Kerstin Bystricky.
Real-time imaging of specific genomic loci in eukaryotic cells using the ANCHOR DNA labelling system
METHODS
2018 May https://doi.org/10.1016/j.ymeth.2018.04.008 - Bonnet J, Durmort C, Jacq M, Mortier-Barrière I, Campo N, VanNieuwenhze M, Brun Y, Arthaud C, Gallet B, Moriscot C, Morlot C, Vernet T, Di Guilmi AM..
Peptidoglycan O-acetylation is functionally related to cell wall biosynthesis and cell division in Streptococcus pneumoniae.
Molecular Microbiology
2017 Dec - Bergé, M. Mercy, C. Mortier-Barriere, I. VanNieuwenzhe, M. Brun, Y. Grangeasse, C. Polard, P. Campo, N. .
A programmed cell division delay preserves genome integrity during natural genetic transformation in Streptococcus pneumoniae.
Nature Communications
2017 Nov - Marie, L. Rapisarda, C. Morales, V. Bergé, M. Perry, T. Soulet, A-L. Gruget, C. Remaut, H. Fronzes, R. Polard, P. .
Bacterial RadA is a DnaB-type helicase interacting with RecA to promote bidirectional D-loop extension
Nature Communications
2017 May - Marie, L. Rapisarda, C. Morales, V. Bergé, M. Perry, T. Soulet, A-L. Gruget, C. Remaut, H. Fronzes, R. Polard, P..
Bacterial RadA is a DnaB-type helicase interacting with RecA to promote bidirectional D-loop extension.
Nature Communications
2017 May - Biondini M, Sadou-Dubourgnoux A, Paul-Gilloteaux P, Zago G, Arslanhan M, Waharte F, Formstecher E, Hertzog M, Yu J, Guerois R, Gautreau A, Scita G, Camonis J, Parrini M..
Direct interaction between exocyst and Wave complexes promotes cell protrusions and motility.
Journal of Cell Science
2016 Oct - Du W, Dubarry N, Passot F, Kamgoué A, Murray H, Lane D, Pasta F..
Orderly Replication and Segregation of the Four Replicons of Burkholderia cenocepacia J2315.
PLoS Genetics
2016 Jul - Johnston C, Hauser C, Hermans PW, Martin B, Polard P, Bootsma HJ, Claverys JP..
Fine-tuning of choline metabolism is important for pneumococcal colonization.
Molecular Microbiology
2016 Jun - Johnston C, Bootsma HJ, Aldridge C, Manuse S, Gisch N, Schwudke D, Hermans PW, Grangeasse C, Polard P, Vollmer W, Claverys JP..
Co-Inactivation of GlnR and CodY Regulators Impacts Pneumococcal Cell Wall Physiology.
PLoS One
2015 Apr - Johnston C, Mortier-Barrière I, Granadel C, Polard P, Martin B, Claverys JP..
RecFOR is not required for pneumococcal transformation but together with XerS for resolution of chromosome dimers frequently formed in the process.
PLoS Genetics
2015 Jan - Johnston C, Martin B, Fichant G, Polard P, Claverys JP..
Bacterial transformation: distribution, shared mechanisms and divergent control.
Nature Reviews Microbiology
2014 Mar - Johnston C, Campo N, Bergé MJ, Polard P, Claverys JP. .
Streptococcus pneumoniae, le transformiste.
Trends in Microbiology
2014 Mar - Johnston C, Martin B, Polard P, Claverys JP..
Postreplication targeting of transformants by bacterial immune systems?
Trends in Microbiology
2013 Oct - Johnston C, Caymaris S, Zomer A, Bootsma HJ, Prudhomme M, Granadel C, Hermans PW, Polard P, Martin B, Claverys JP..
Natural genetic transformation generates a population of merodiploids in Streptococcus pneumoniae.
PLoS Genetics
2013 Sep - Berge MJ, Kamgoué A, Martin B, Polard P, Campo N, Claverys JP..
Midcell recruitment of the DNA uptake and virulence nuclease, EndA, for pneumococcal transformation.
PLoS Pathogens
2013 Sep - Johnston C, Polard P, Claverys JP..
The DpnI/DpnII pneumococcal system, defense against foreign attack without compromising genetic exchange.
Mobile Genetic Elements
2013 Jul - Johnston C, Martin B, Granadel C, Polard P, Claverys JP. .
Programmed protection of foreign DNA from restriction allows pathogenicity island exchange during pneumococcal transformation.
PLoS Pathogens
2013 Feb - Mirouze N, Berge MA, Soulet AL, Mortier-Barriere I, Quentin Y, Fichant G, Granadel C, Noirot-Gros MF, Noirot P, Polard P, Martin B, Claverys JP..
Direct involvement of DprA, the transformation-dedicated RecA loader, in the shut-off of pneumococcal competence.
PNAS
2013 Feb - Martin B, Soulet AL, Mirouze N, Prudhomme M, Mortier-Barrière I, Granadel C, Noirot-Gros MF, Noirot P, Polard P, Claverys JP..
ComE/ComE~P interplay dictates activation or extinction status of pneumococcal X-state (competence).
Molecular Microbiology
2013 Jan - Quevillon-Cheruel S, Campo N, Mirouze N, Mortier-Barriere I, Brooks MA, Boudes M, Durand D, Soulet AL, Lisboa J, Noirot P, Martin B, van Tilbeurgh H, Noirot-Gros MF, Claverys JP, Polard P..
Structure-function analysis of pneumococcal DprA protein reveals that dimerization is crucial for loading RecA recombinase onto DNA during transformation.
PNAS
2012 Sep - Charpentier X, Polard P, Claverys JP..
Induction of competence for genetic transformation by antibiotics: convergent evolution of stress responses in distant bacterial species lacking SOS?
Current Opinion in Microbiology
2012 Aug - Attaiech L, Olivier A, Mortier-Barriere I, Soulet AL, Granadel C, Martin B, Polard P, Claverys JP. .
Role of the single-stranded DNA-binding protein SsbB in pneumococcal transformation: maintenance of a reservoir for genetic plasticity.
PLoS Genetics
2011 Jun - Costes A, Lecointe F, McGovern S, Quevillon-Cheruel S, Polard P..
The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.
PLoS Genetics
2010 Dec
